Biology Reference
In-Depth Information
(a)
(b)
Fig. 3.
Stereo pairs of tilted side views of the reconstructed N-trimer show-
ing the docking of three double-stranded RNA (dsRNA), each 20 nucleotides
(nt) long (
a
), and three-single stranded RNA (ssRNA), each 12 nt long
(
b
) to the three symmetrically joint cavities of the trimer. The preferred loca-
tions for dsRNA and ssRNA in the trimer were found by performing the RNA
docking with an inverted 3D electron density map of the trimer (inverted 3D
reconstructions would represent the hollow space in and around the trimer).
SITUS was used for docking (http://situs.biomachina.org/), and VMD was
used for visualization of stereo pairs of the docked RNAs with the density
maps of N-protein as suggested in SITUS.
