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route and target cells. 6,31 Third, HEV-VLPs are stable at room tem-
perature. Fourth, anti-HEV immune responses had no effect on
boosting administration in the present study. Thus, HEV-VLPs are an
attractive vaccine vector in developing countries because these VLPs
can be preserved without the requirement of any particular equip-
ment. These findings suggest that chimeric VLPs derived from orally
transmissible viruses can be used as vaccine vectors to mucosal tissue
by oral administration for the purpose of vaccination.
Acknowledgments
All experiments described in this chapter were carried out by
Dr. Masahiro Niikura (Department of Microbiology and Molecular
Genetics, Michigan State University) and Dr. Shiki Takamura
(Department of Bioregulation, Mie University School of Medicine,
Mie, Japan). This work was supported by Health Science Research
Grants from the Ministry of Health, Labor and Welfare of Japan; the
Ministry of Education, Culture, Sports, Science and Technology of
Japan; and Regional Science Promotion Program.
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