Biology Reference
In-Depth Information
Intestinal fluids
Sera
OD
OD
OD
OD
A. IgG
B. IgA
C. IgG
D. IgA
1.6
1
0.2
0.16
0.8
0.16
1.2
0.12
0.6
0.12
0.8
0.08
0.4
0.08
0.4
0.04
0.2
0.04
0
0
0
0
24 68
weeks pi
24 68
weeks pi
24 68
weeks pi
24 68
weeks pi
Fig. 6.
IgG (A and C) and IgA (B and D) levels in intestinal fluids (A and B)
and sera (C and D) of orally immunized mice. Circles and triangles indicate
HEV-specific and the tag epitope-specific antibody levels, respectively, in
individual mice. Two immunized mice were sacrificed at each time point
(two, four, six and eight wpi). Specific antibody levels to HEV and the tag
epitope of control mice immunized with VLP without the tag (closed circles
and closed triangles, respectively) and background levels to HEV and the tag
epitope of non-immunized mice (squares and crosses, respectively) are also
shown. Antibody levels are indicated as OD405 in ELISA when sera and
intestinal fluids were diluted at 1:100 and 1:2, respectively.
subclasses of IgGs to HEV — except IgG3, IgM, and IgA — were evi-
dent in all mice (Fig. 7B). Both to the tag and HEV, all mice failed to
develop IgG3 above the detectable level (Figs. 7A and 7B). In the con-
trol mice immunized with VLPs without the tag, HEV-specific anti-
body reactions similar to the those with the chimeric VLPs were shown
(Fig. 7B), while no detectable level of any isotype antibody specific to
the tag was observed (Fig. 7A), as expected.
Induction of foreign epitope-specific antibody (Ab) responses by
chimeric VLP administration is not easy compared with inducing cellu-
lar immune responses such as a cytotoxic T lymphocyte (CTL)
response.
8,10,11,21,22
Moreover, our results showed Ab responses by oral
