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Fig. 8. Distribution of electrostatic potential on a P8-trimer, as viewed from
the interface between the core and the outer shell and between P8-trimers. 21
Positively charged regions are colored blue and negatively charged regions
are colored red. The atomic model of the P8-trimer of RGDV (b) was based
on that of the P8-trimer of RDV (a); figures were prepared with GRASP. 27
surface of the P8 protein, namely, on the surface of the virus. Further-
more, the structure of the P8-trimer of RGDV, constructed on the
basis of the atomic structure of the P8-trimer of RDV, had a similar
distribution of electrostatic potential to that of the bottom of the
P8-trimer of RDV (Fig. 8, top panels), which is the region that is
assumed to play a critical role in the interaction between the P8-trimer
and the three-fold axes of core particles. 2 The two kinds of similarity
on the lower part of the P8-trimer, which binds to the core particle,
suggested that it might be possible for P8-trimers of RGDV to bind
at the three-fold axes of RDV core particles, i.e. via the vertical inter-
actions of heterologous proteins. The validity of this suggestion was
confirmed by the demonstration that treatment with 0.4 M MgCl 2
of core particles prepared from chimeric virus-like particles, obtained
by co-expression of P3 of RDV and P8 of RGDV, contained relatively
small amounts of RGDV P8 protein, which were resistant to treatment
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