Biology Reference
In-Depth Information
Table 8. Engineered pRNA ring set to argue the pentameric pRNA ring the-
ory, that are “Four Plus One” and “Six Minus One,” respectively. The number
inside ring represented yield of infectious virions (PFU/ml) in
in vitro
assem-
bly assays (Shu, Zhang and Guo, manuscript in preparation)
“Six Minus One”
Another set, “Six Minus One,” to compose a five-member pRNA
ring, was accomplished by incorporating one inactive mutant dimer
pRNA to replace one member of the two trans-complementary dimer
pRNAs — that is, the “Three Dimer Set” (Table 8). For example,
mixing two dimers with active (
A-b´
)(
B-a´
) and one mutant dimer