Biology Reference
In-Depth Information
Fig. 8.
(
a
) Human immunodeficiency virus (HIV) particles isolated from
culture medium. (
b
) The surface of an infected H9 lymphocytic cell is cov-
ered with a cascade of HIV particles. (
c
) High-resolution AFM image of an
HSV virion on an H9 cell surface. Clusters of envelope proteins seem to
arise from more or less arbitrary associations of subunits. Average particle
diameter is 127 nm.
and shape of tufts along with the absence of threefold symmetry as seen
in AFM images suggest that in the native HIV particle, gp120 may
form arbitrary clusters rather than closely associated symmetric units.
Acknowledgments
The authors thank E. K. Wagner, P. D. Gershon, H. Fan, W. E. Robinson,
S. Sandmeyer and J. V. Van Etten for virus preparations and helpful
discussions. We wish to thank A. Greenwood, R. Lucas, J. Zhou, M. K.
Rice for technical assistance. The National Aeronautics and Space
Administration and National Institute of Health supported this research.
Work was performed under the auspices of the US Department of
Energy by the University of California, Lawrence Livermore National
Laboratory, under contract W-7405-ENG-48.
References
1.
Harrison SC. (1990) Principles of virus structure. In B.N. Fields and
D.M. Knipe (eds),
Fields Virology
, 2nd edn., pp. 37-61. Plenum Press,
New York.
