Biology Reference
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G*G, lacking VSVG gene
but possessing the VSVG protein on the viral particles, infects to cells
expressing E1 and E2 proteins. After removing unbound viruses, infected
cells are cultured for one day and pseudotype VSVs are recovered. If target
cells had a receptor for the pseudotype viruses, the viruses could infect and
express green fluorescent protein (GFP).
Fig. 3.
Construction of pseudotype VSVs. VSV
∆
recombinant VSVs possessing chimeric HCV envelope proteins to var-
ious mammalian cell lines has been reported by Rose and colleagues,
14
molecular size of the chimeric E1 protein incorporated into the recom-
binant VSVs is smaller than that observed in the pseudotype VSVs.
Therefore glycosylation or other post-translational modifications of
HCV envelope proteins expressed by different systems or cell lines
might determine the infectivity of recombinant VSVs.
Pseudotype particles based on MLV or HIV possessing unmodified
HCV envelope proteins have been generated.
9,44
Although HCV enve-
lope proteins are believed to retain in the ER,
20
Bartosch
et al
. and
another group reported that the native form of HCV glycoproteins were
partially expressed on cell surface of 293T cells and encapsulated retro-
virus nucleocapsid, resulting in the production of infectious particles.
9,44
These pseudotype particles produced by transfection of 293T cells with
