Biomedical Engineering Reference
In-Depth Information
Followed by the preparation and characterization of the cross-linked biopolymer
material using collagen and AA.
CoNClusioN
In the present study the choice for the preparation of thermally stable and mechani-
cally stable biopolymer material was Type I and Type III collagen of bovine skin and
avian intestine with a natural polymer AA.The reason for the selection of Type I and
Type III collagen was due to its interaction with number of cells and its involvement
in most of the human and animal diseases. Though numbers of studies were available
for the different use of AA, its cross-linking potential with collagen has not yet in re-
ports.The present study describes, the cross-linking ability and chemistry of collagen
with AA was assessed using FT-IR, TGA, and degree of cross-linking measurements
using standard procedures. The results obtained and the schematic representation of
the reaction mechanism summarized proves, AA was cross-linked with collagen in
two pathways one through multiple intermolecular hydrogen bonding (proved by bio-
informatics study) and second is by covalent linkage (proved by TNBS/percentage of
cross-linking degree assay).The biopolymer material (sheet) prepared upon cross-link-
ing of AA was the green method of preparation. No toxic compounds were involved in
this preparation and the resultant material found application as wound dressing sheet
in clinical applications.
KeyWords
•
acid soluble collagen
•
alginic acid
•
anionic polysaccharides
•
Collagen
•
Cross-linking
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