Cross-linking Chemistry of Type I and Type III Collagen with Anionic Polysaccharides - Natural Polymers, Biopolymers, Biomaterials, and Their Composites, Blends, and IPNs - page 165

AACC 3. In general, the amide I, II and, III peaks of Type I collagen was located at
1,658, 1,556, and 1,240 cm -1 . However, after cross-linking with AA, a sharp intense
peak at 1,658 disappears with the overlapping peak of -C=O and -N-H bands in the
range of 1,600-1,650 cm -1 in AACC 1 were observed. This might be due to the forma-
tion of covalent linkage between -COOH group of AA and ε-NH 2 group of collagen
(Pavia et al., 2001). In addition, deep and sharp intense peak observed at 1,556 cm -1 in
Type I collagen and the related overtone of 1,553 at 3,081 cm -1 was reduced without
any overtone peaks in AACC 1. Simultaneously, the intensity of amide III was also
reduced in AACC 1 compare to Type I collagen. However, in Type III collagen the
amide I, II and III peaks were located at 1,655, 1,546, and 1238 cm -1 and after cross-
linking with AA these three sharp amide signals have been reduced due to the above
said reason. In addition, one significant change was observed for Type III collagen,
that is, the overtone peak at 2,924 cm -1 which, was remarkably reduced in AACC 3
spectrum, suggests, the interaction of both types (Type I and Type III) of collagen with AA.
Figure 3. Illustrate, FT-IR spectral details of alginic acid, Type I collagen, Type III collagen, AACC 1
and AACC 3.