Biomedical Engineering Reference
In-Depth Information
PreParatioN oF Cross-liNKed BioPolymer material
Different concentrations (0.5, 1.0, 1.5, 2.0, 3.0, 4.0, and 5.0%) of homogenized solu-
tion of AA were prepared by dissolving the required quantities in 70 (mM) sodium
phosphate buffer (pH 6.5) under stirring for overnight at room temperature. About
0.5% (w/v) RASC (dissolved in 0.005M acetic acid) was mixed with different con-
centration of AA at 3:1 (RASC:AA) ratio respectively and the homogenized solution
obtained upon stirring for 30 min at room temperature, incubated for overnight at 4ºC.
Followed by incubation, the reaction mixture was then transferred to a polypropylene
plate (Tarson, India) and air-dried at 37°C for 12 hr. The biopolymer material obtained
in the form of sheets from the above process was designated as AA cross-linked Type I
(AACC 1) and Type III collagen (AACC 3). In addition, a separate collagen sheet ma-
terial without cross-linker was also made accordingly and used for comparative analy-
ses. The dried polymer sheets were further subjected to percentage of cross-linking
degree, FT-IR, TGA, and Tensile strength analyses.
Figure 2 demonstrated, degree of cross-linking measurements (Bubnis and Ofner,
1992) of AA cross-linked biopolymer material with increasing concentration of AA.
Maximum degree of cross-linking of 75% was observed with 1.5% concentration of
AA with 0.5% of either Type I or Type III collagen. No further increase in degree of
cross-linking with increasing concentration of AA was observed.
Figure 2.
Degree of cross-linking measurements.
The FT-IR studies were conducted to monitor the chemical modifications in col-
lagen structure due to cross-linking with AA.
Figure 3
illustrate, FT-IR spectral details
of AA, Type I collagen, Type III collagen, AACC 1, and AACC 3. When comparing
the FT-IR spectral details we observed a complete different spectrum for AACC 1 and
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