Cross-linking Chemistry of Type I and Type III Collagen with Anionic Polysaccharides - Natural Polymers, Biopolymers, Biomaterials, and Their Composites, Blends, and IPNs - page 163

Flow chart for extraction of Collagen from Avian intestine
Avian intestine obtained from slaughter house
Washed and cleaned the waste materials inside the intestine
Washed with chloroform for complete removal of fat materials
Chopped in to pieces
Soaked in 0.5(M) Sodium acetate buffer with continuous stirring at 4ºC for 12-24h
Homogenized with 0.5 (M) Acetic acid
Centrifuged at 10,000rpm for 20 min at 4ºC
Supernatant precipitated with 5% NaCl
Centrifuged at 10,000rpm for 20 min at 4ºC
Residue collected & Re-dissolved in 0.05(M) acetic acid
Dialyzed against 0.005(M)acetic acid
Lyophilized
Acid soluble collagen
The molecular profile of Type I and Type III collagen were shown in Figure 1(a)
and 1(b), and it demonstrate, presence of two types of α chains (α 1 and α 2 ) (100Kda)
and β dimer (200 Kda) in Type-I collagen and one type of α chain (α 1 ) (100 KDa) and
β dimer (200 KDa) for Type-III collagen ( Kuga et al., 1998; Lin and Liu, 2006).
Figure 1. The molecular profile of (a) Type I and (b) Type III collagen.