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conducted over relatively long distances of up to 25 m in the laboratory. Chapters 4 and 5 form
part II of the thesis. In Chapter 4 , both the intra-strain and inter-strain heterogeneities were
studied over a distance of 5 m in two solutions of different ionic strengths. The minimum
sticking efficiency method developed in Chapter 4 was applied in Chapter 5 to study the
transport of two environmental E. coli isolates.
In part III , methods to determine the cell characterization and transport of E. coli strains in Parts
I and II were applied to study and measure cell properties and transport characteristics of E. coli
strains isolated after they have been transported through aquifers. Chapters 6 and 7 form Part III .
In chapter 6 , inter-population and intra-population heterogeneities commonly observed with E.
coli strains isolated from different sources were investigated using spring E. coli isolates, and
chapter 7 focuses on bacteriological and physicochemical analyses of springs in the Lubigi
catchment in Kampala, Uganda, and the transport of 40 E. coli strains isolated from the springs.
The chapter also highlights on the variability in phenotypic characteristics of selected E. coli
strains.
Summaries and conclusions of the research findings are presented in Chapter 8 which is the last
part ( Part IV ) of this thesis.
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