Biomedical Engineering Reference
In-Depth Information
Table 8
Capture probes and analytes for the analysis of microbead surface reactions
Target
Capture probe
Analyte
VIM-cap
p: bt-ACCTGCTCAATGTTAAGATGGCCCTTGA
CATTGAGATTGCC
D: BHQ2-ACCTACAGGAAGCTGCTGG-P
CCAGCAGCTTCCTGTAGGTgGC
AATCTCAATGTCAAGGGCCATCTT
AACATTGAGCAGGT
GAPDH-cap
p: NH
2
-(dT)
10
TGCACCACCAACTGCTTAGC
GCTAAGCAGTTGGTGGTGCA
MLC-2v-cap
p: NH
2
-(dT)
20
TCGAACCCTTGCCACTCATCTTCC
CCAAGGGCAGCAAAGGTGTCTCT
poly(dA)
20
-cap
p: NH
2
-AAAAAAAAAAAAAAAAAAAA
TTTTTTTTTTTTTTTTTTTT
aCS-cap
p: NH
2
-(dT)
20
GAAATGAGAGAGGCCCAGCTACTGC
GCAGTAGCTGGGCCTCTCTCATTTC
HPRT1-cap
p: NH
2
-(dT)
20
CCTTTTCACCAGCAAGCTTGCGACCT
AGGTCGCAAGCTTGCTGGTGAAAAGG
SERCA2-cap
p: NH
2
-(dT)
20
TCGAACCCTTGCCACTCATCTTCC
GGAAGATGAGTGGCAAGGGTTCGA
D: Detector probe (compare Fig.
16
) for VIM. The capture probe (p) was 5
0
amino (NH
2
) modified or biotinylated (bt) with an additional poly(dT)
10/20
region
