Biomedical Engineering Reference
In-Depth Information
A Highly Versatile Microscope Imaging
Technology Platform for the Multiplex
Real-Time Detection of Biomolecules
and Autoimmune Antibodies
Stefan Rödiger, Peter Schierack, Alexander Böhm, Jörg Nitschke,
Ingo Berger, Ulrike Frömmel, Carsten Schmidt, Mirko Ruhland,
Ingolf Schimke, Dirk Roggenbuck, Werner Lehmann
and Christian Schröder
Abstract The analysis of different biomolecules is of prime importance for life
science research and medical diagnostics. Due to the discovery of new molecules
and new emerging bioanalytical problems, there is an ongoing demand for a
technology platform that provides a broad range of assays with a user-friendly
flexibility and rapid adaptability to new applications. Here we describe a highly
versatile microscopy platform, VideoScan, for the rapid and simultaneous analysis
of various assay formats based on fluorescence microscopic detection. The tech-
nological design is equally suitable for assays in solution, microbead-based assays
and cell pattern recognition. The multiplex real-time capability for tracking of
changes under dynamic heating conditions makes it a useful tool for PCR appli-
cations and nucleic acid hybridization, enabling kinetic data acquisition impossible
to obtain by other technologies using endpoint detection. The paper discusses the
technological principle of the platform regarding data acquisition and processing.
Microbead-based and solution applications for the detection of diverse biomole-
cules, including antigens, antibodies, peptides, oligonucleotides and amplicons in
S. Rödiger
P. Schierack
A. Böhm
J. Nitschke
I. Berger
U. Frömmel
C. Schmidt
M. Ruhland
C. Schröder (
)
Lausitz University of Applied Sciences, Senftenberg Germany
e-mail: Christian.Schroeder@HS-Lausitz.de
W. Lehmann
Attomol GmbH, Lipten Germany
e-mail: werner.lehmann@attomol.de
D. Roggenbuck
MEDIPAN GmbH, Berlin Germany
e-mail: D.Roggenbuck@Medipan.de
S. Rödiger
I. Schimke
Medizinische Klinik (Kardiologie),
Charité-Universitätsmedizin Berlin, Berlin Germany
e-mail: Ingolf.Schimke@charite.de
&
