Biomedical Engineering Reference
In-Depth Information
effects. In both cases, the measurement for that spot will be inaccurate. Local
artefacts can increase the local background and thus reduce the intensity of the
signal resulting in lower protein quantification. Over shining effects can have an
effect on the positioning of the grid and thus increase the variance of the same
protein at replicate experiments. To overcome these problems the grid and radius
can be fitted manually to each individual spot which takes a lot of time and is
prone to errors. Much more efficient is an automated detection of distortions on the
protein microarray and the labelling of the artefacts and over shining effects for the
subsequent evaluation process. Figure 12 shows intensity line diagram generated
with software sciANA 4.1. The green intensity plot displays the signal intensities
of 10 with identical peptide concentration of equal spotting volume along the line
given in the inverted b/w picture.
6 Conclusion
In contrast to closed systems for biochips that have been promoted for a number of
years and been successful (e.g UNIgene Arrays from Protagen (Dortmund,
Germany), IgXPLEX TM celiac qualitative assay from SQI Diagnostics (Toronto,
Canada) the approach described here offers selectable combinations of state of the
art technologies for the production and analysis of microarrays with any content in
scalable formats at significantly reduced manufacturing costs. This toolbox
approach allows for any adoptions to individual needs and formats that are scalable
from research and development to diagnostics manufacturing purposes without a
technology switch—where the presented non-contact printing technology, the
consumables and detectors covers many aspects of the technology, but needs to
work
hand
in
hand
with
surface
chemistry,
assay
development,
alternative
detection technologies and algorithms for data analysis.
References
1. Vinayagam A, Stelzl U, Foulle R, Plassmann S, Zenkner M, Timm J, Assmus HE, Andrade-
Navarro MA, Wanker EE (2011) A directed protein interaction network for investigating
intracellular signal transduction. Sci Signal 4(189):rs8
2. Haab BB, Yue T (2011) High-throughput studies of protein glycoforms using antibody-lectin
sandwich arrays. Methods Mol Biol 785:223-236
3. Eickhoff H, Konthur Z, Lueking A, Lehrach H, Walter G, Nordhoff E, Nyarsik L, Büssow K
(2002) Protein array technology: the tool to bridge genomics and proteomics. Adv Biochem
Eng Biotechnol 77:103-112
4. Walter G, Büssow K, Lueking A, Glökler J (2002) High-throughput protein arrays: prospects
for molecular diagnostics. Trends Mol Med 8(6):250-253
5. Büssow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach H, Walter G (1998) A
method for global protein expression and antibody screening on high-density filters of an
arrayed cDNA library. Nucleic Acids Res 26(21):5007-5008
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