Biomedical Engineering Reference
In-Depth Information
Planar Protein Arrays in Microtiter
Plates: Development of a New Format
Towards Accurate, Automation-Friendly
and Affordable (A
3
) Diagnostics
Holger Eickhoff and Arif Malik
Abstract A technology for protein microarrays in 96 well microplates has been
developed as a widely adoptable platform for multiplexed protein based diag-
nostics. Procedures for protein microarray manufacturing, immobilization, assay
optimization and optical detection methods were developed. Using a clever
combination of state of the art technologies, a versatile platform that works with
peptides, proteins and antibodies as capture agents in a scalable format for planar
arrays is described.
Keywords Biosensor
Diagnostics
Microarray
Non-contact
spotting
Test format
Contents
1
Introduction........................................................................................................................
150
2
Substrates for Protein Binding ..........................................................................................
150
3
Direct Printing into Microplate Wells ..............................................................................
151
3.1
Contact Printing Versus Non-Contact Printing .......................................................
152
3.2
Microarrays with Up to 1400 Spots Per Well.........................................................
155
4
Colorimetric Protein Array Detection ..............................................................................
157
4.1
Detection Devices for Colorimetric Array Readout................................................
161
5
Sophisticated Software Tools are Needed for Array Production and Analysis..............
161
5.1
Determination of Spot Position and Grid Alignment .............................................
162
5.2
Determination of Spot Intensity ...............................................................................
163
6
Conclusion .........................................................................................................................
164
References................................................................................................................................
164
H. Eickhoff (
)
Scienion AG, Volmerstr. 7b, 12489 Berlin, Germany
e-mail: eickhoff@scienion.com
A. Malik
Microdiscovery GmbH, Marienburger Strasse 1, 10405 Berlin, Germany
e-mail: arif.malik@microdiscovery.de
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