Biology Reference
In-Depth Information
Figure 14.
Photomicrographs of the skeletal muscle of spontaneously metamorphosing
Japanese fl ounder. Left and right columns show longitudinal and transverse sections,
respectively. (a,b) Premetamorphosis; (c,d) Prometamorphosis; (e,f) Post climax [From Yamano
et al., 1991, with permission].
chains and three major light chains: two alkali light chains and one 5,5'-
dithio-bis-nitrobenzoic acid (DTNB) light chain. During metamorphosis,
the larval type DTNB light chain is gradually replaced by the adult type
DTNB light chain, whereas the heavy chains and two alkali light chains do
not change throughout metamorphosis. Troponin-T isoforms also change
during metamorphosis. The skeletal muscle of premetamorphic larvae
has two immunoreactive troponin-T isoforms of 41.5 and 34.0 kDa, with
the former being predominant. At the metamorphic climax a new isoform
appears at 33.5 kDa, and the two isoforms, 33.5 kDa and 34 kDa, become
predominant.
Such morphological development and transition of molecular
constituents of the muscle during ontogeny have also been reported for
