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Figure 12. Photomicrographs of the gastric wall of spontaneously metamorphosing
Japanese fl ounder at three different developmental stages. (a) Premetamorphosis. (b) early
prometamorphosis. Note primordial crypts (arrowheads). (c) post-climax. L, gastric lumen;
G, gastric glands. (a-c) Same magnifi cation. [From Miwa et al., 1992, with permission].
crypts. The cells at the bottom of the crypt proliferate and form simple
alveolar or acinar structures (Fig. 12-b). At metamorphic climax, the
glandular cells rapidly proliferate, resulting in developed gastric glands
at post climax stage (Fig. 12-c). The gastric wall of premetamorphic larvae
shows no immunohistochemical reaction with antiserum to pepsinogen,
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