Biology Reference
In-Depth Information
Figure 8.
1a-c,
Midsagittal sections of the pituitary of Japanese fl ounder larvae treated by
0.05 ppm T
4
or 30 ppm TU for 2 weeks, stained with anti-hTSHβ. Anterior direction is left.
(a)Control fi sh. Ir TSH cells show the features of prometamorphic larvae. (b) Fish treated
with T4. Ir TSH cells are not evident. (c) Fish treated with TU. The number of Ir TSH cells is
markedly increased. H, Hypothalamus; N, neurohypophysis; PPD, proximal pars distalis;
RPD, rostral pars distalis. a-c ×886.
2a-c,
Sagittal sections of thyroid gland of fl ounder larvae treated by 0.05 ppm T4 or 30 ppm
TU for 2 weeks, stained with anti-T4 serum, conterstained with methyl green. (a)
Control
fi sh. The follicular cells are cuboidal and the colloid shows variable staining intensity with
the antiserum (arrowheads). (b) Fish treated with T4. Follicular epithelium is fl attened, and
the colloid is only weakly stained with the antiserum.
(c) Fish treated with TU. The follicular
cells are markedly taller, and the follicles contain only a small amount of colloid which shows
no reactivity to antiserum (arrowheads). C, cartilage. a-c ×738. [From Miwa and Inui, 1987a,
with permission].
in rainbow trout (Iuchi, 1973a; Yamamoto and Iuchi, 1975) and angelfi sh
(Al-Adhami and Kunz, 1976).
The Japanese fl ounder also shows a developmental shift of erythrocyte
populations from primitive to defi nitive erythrocytes during metamorphosis
