Skeletal Stem Cells - Engineered Bone: Tissue Engineering Intelligence Unit - page 14

Table 1. Phenotypic characterization of multi-colony derived strains of human
marrow SSCs and guinea pig blood-borne adherent cells, and of
single-colony derived strains of human and rabbit blood-borne adherent cells

Rabbit Human Human

Rabbit Fibroblast- Fibroblast- Fibroblast- Human

Polygonal Like Guinea Pig Like Like Marrow

Marker* Nonosteogenic Osteogenic Osteogenic Nonosteogenic Osteogenic SSCs

FIBROBLASTIC
Col I ++ + ++ ++ ++ ++

Col III -/+ -/+ + + + ++

Fibronectin ++ ++ ++

CD-44 (H9H11) ++ ++ ++

β 1 integrin subunit ++ +/++ ++

OSTEOGENIC
ON ++ +/++ ++ -/+ ++ ++

OPN +/++ + ++ -/+ -/+ -/+

BSP +/++ + + - - -/+

OC -/+ -/+ -/+

ADIPOCYTIC
CEBP α -/+ -/+ -/+

PPAR γ 2 - - - - -

ENDOTHELIAL
CD34 - - -

Muc-18 - - +

Factor VIII - - -

Endoglin - - +

PAL-E - -

EN4 -

MARROW STROMAL CELLS
Stro-1 - - -/+

VCAM-1 ++ ++ +

SMOOTH MUSCLE
α -smooth muscle actin + ++ ++ ++ ++ -/+/++

Smooth muscle -/+ + +/++

ACL-10002
HISTOCHEMICAL
Alkaline Phosphatase - - - - - -/+/++

Acid Phosphatase + + + + + +

α -Naphthyl acetate - - +/++ +/++ +

esterase
Oil Red O (Lipid Accumulation)
20% Fetal Bovine -/+ - -/+ -/+ + -/+

Serum
20% Rabbit Serum +/++ -/+/++ ++ ++ ++ ++

*Markers for leukocytes, monocytes/macrophages, skeletal muscle, and neuronal cells were negative
in all cell types studied. ++ intensive staining; + weak staining; - negative staining

artificial inducing stimuli, 30,53,59,62 and these data need to be corroborated by physiologically
relevant in vivo observations. In other studies, analogous assertions were based exclusively on
improved clinical outcome, with no specific markers proving SSC differentiation towards