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Figure 9: Semiquantitative RT-PCR reveals the transcriptional dynamics of spkC, spkD, spkF and spkG at different conditions.
The transcript level of RNase P in each sample serves as a control. N, normal conditions; LT, low temperature; HT, high
temperature; HS, high salt; -C, carbon-defi cient; -N, nitrogen-defi cient; -P, phosphorus-defi cient. With the kind permission
of S. Qin, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. [Liang et al . (2011) PLoS ONE 6(5): e18718.
doi:10.1371/journal.pone.0018718] doi:10.1371/journal.pone.0018718.g001
Gene sequences governing the synthesis of Group 3 sigma factors SigH (sll0856), SigG (slr1545)
and SigF (slr1564) and the regulatory protein RsbU (slr-2031) have been identifi ed in the genome
of Synechocystis sp. strain PCC 6803 and subjected to interposon mutagenesis to generate mutants
to understand their role in stress tolerance. Mutant SigF showed a severe defect in the induction of
salt-stress proteins. Though its ability to tolerate salt stress (up to 684 mM NaCl) was not apparently
changed but its ability to tolerate still higher concentrations of salt was reduced. A comparison of wild-
type and Sig mutants revealed no differences in their growth rates, pigment contents, photosynthetic
and respiratory activities and the level of accumulation of GG (Huckauf et al ., 2000).
vii) Common stress mechanisms : The genome of A . halophytica contains a gene DnaK1 that encodes a
polypeptide of 721 amino acid residues with a C-terminal tail composed of many acidic amino acids.
In order to understand whether this protein has a chaperone like activity and the role of C-terminal
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