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Figure 3: Heterocyst differentiation in microelectromechanical system-assisted liquid microchambers. Spatiotemporal
dynamics in morphological changes (BF, bright fi eld), hetR promoter (P hetR ) activity monitored with a transcriptional gfp
fusion reporter ( hetR ), and phycobilisome fl uorescence (Pbs) in an individual Anabaena fi lament at the indicated times after
nitrogen step-down. The dashed square at the top indicates the space of a microchamber. The bottom panel (taken at 70 h)
shows a magnifi cation of part of the fi lament. With the kind permission of H. Iwasaki, Department of Electrical Engineering
and Biological Science, Waseda University (TWIns), Tokyo, Japan & PRESTO, Japan Science and Technology Agency (JST),
Tokyo, Japan [Asai et al . (2009) PLoS ONE 4(10): e7371. doi:10.1371/journal.pone.0007371.g001].
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