Biomedical Engineering Reference
In-Depth Information
and microvilli to enhance cell-environment exchange. The results show that the films
deposited at −600 V have a higher sp
2
/sp
3
fraction, and 600CD4 has the highest polar com-
ponent of the surface energy. The actin labeling with FITC-phalloidin reveals the cytoskel-
etal organization in Figure 2.34.
The attachment and proliferation of MG63 osteoblast-like cells were studied on
Si-incorporated unhydrogenated a-C [107] and Si- and SiO
x
-incorporated hydrogenated
a-C [120]. Although substantial differences exist in surface and bulk compositions between
these films (a-C:H, a-C:H(Si) and a-C:H(SiO
x
)), osteoblast adhesion did not show trends
in cell numbers, cell areas, or cell morphologies that can be attributed to either the Si/
SiO
x
content of the film or to the surface chemical structure of the films after a 3-day
Ctrl
Si
400CD4
400CH4
400CD4/CH4
600CD4
600CH4
600CD4/CH4
FIGURE 2.34
Cytoskeleton assessment MC3T3-E1 osteoblasts after a 3-day culture. All coatings induce a similar intensity
and organization of actin stress fibers. (Reprinted with permission from Chai et al.,
Acta Biomater.
, 4, 1369,
2008.)
