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Manoalide 3318 together with a steroid, isolated from a marine sponge
Smenospongia sp., showed an antifouling activity but no toxicity against
the cypris larvae of a barnacle Balanus amphitrite ,withanEC 50 value of
0.24
gmL -1 ) [13].
10-Formamido-4-cadinene 3319 obtained from a sponge, Acanthella caver-
nosa , also inhibited a larval settlement at a concentration of 0.5
gmL -1 (CuSO 4 : 0.15
µ
µ
gmL -1 [14].
The sixteen secondary metabolites of a green alga Caulerpa prolifera were
isolated. They are shown as compounds C3320 - C3335 .Theantifoulingactiv-
ity of the algal extracts in laboratory assays against two of the major groups
of fouling organisms. The extracts exhibited moderate to significant activi-
ties against two Gram-positive and one Gram-negative marine bacteria. The
measured inhibition zone varied from 17 to 83% of that observed with the
biocide TBTO. On the other hand, the growth inhibitory effect of the Caulerpa
prolifera extract was evaluated against a marine microalga Phaedactylum tri-
µ
Scheme 4
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