Biology Reference
In-Depth Information
Table 15.
Classifi cation of gonads of presumptive male zebrafi sh at 28 dph (compiled from
Wang et al., 2007)
Type
Features
I
EGFP expression too low to be detected. Neither the ovary-like cavity nor the
elongate shape of ovary found before or after transformation. Oocytes are not
detected or hardly with one or two in one sectioned ovary. Comprises 42% of
presumptive males, which directly develop into males
II
Faint EGFP expression. Number of oocytes are few and are less than that
observed in type III. In terms of structure of the ovarian cavity and shape of
ovary, gonads are partially developed. Comprises 32% of presumptive males
III
Normal EGFP expression. Oocytes are detected. Comprises 26% of presumptive
males
al. (2008) for six genes suggest an important role for
ar
,
sox9a
and
Dmrt1
in
controlling male differentiation and
fi g a
and
cyp19a1a
in regulation of female
differentiation (Fig. 16). Evidence from studies on the non conventional
fi sh pejerrey
Odontesthes bonariensis
, in which sex differentiation is known
Fig. 16.
Tissue specifi c expression of
ar
,
sox9a, dmrt1
,
fi gα
,
cyp19ala
and
cyp19alb
from (A) male
and (B) female zebrafi sh. L: liver, Gu: gut, S: spleen, M:muscle, Go: gonad, Gb: gall bladder,
H: heart, Gi: gills, B: brain, E: eye, +: control (from Jorgensen et al., 2008)