Biology Reference
In-Depth Information
Fig. 12.
Expression levels of
Dmy
transcripts at 0 dah in XY
HNI
, XY
Hd-rR
and sex reversed
mutants of
Oryzias latipes.
(A) Ethidium bromide-staining of the
Dmy
transcripts following
gel electrophoresis. β-actin is loaded as a loading control: (B) Columns show the ratio of
Dmy
mRNA β-actin mRNA in arbitrary unit. Astriks indicate statistically signifi cant differences
between the mean values of XY
HNI
and XY
Hd-rR
as well as XY
HNI
XY
Hd-rR
and Y
wKsn
Y
wKsn
(from
Otake et al., 2006, with permission by the Genetic Society of America)
in embryos overexpressing
dmrt1bY
and cells are arrested in the G2 phase
of the cell cycle (Herpin et al., 2007). Hence, the proliferation in male
embryos commenced later than in female embryos, when
Dmrt1bY
acts
in an autocrine manner (Kondo et al., 2009). The proliferation of PGCs is
accelerated from stage 37 in XX embryos and reaches a peak with about
125-225 cells at stage 39. However, in XY embryos, after a brief period of
