Biology Reference
In-Depth Information
Table 9.
Representative examples of molecular markers to identify sex, sex chromosome and
sex determining locus in selected fi shes (from Pandian, 2011, updated)
Species and Reference
Remarks
Xiphophorus maculatus
Coughlan et al. (1999)
PCR based genomic DNA analysis identifi ed the presence of
323bp fragment in XX and YZ females and 323 bp plus high
molecular fragment in XY and YY males
Puntius conchonius
Kirankumar et al. (2003)
SRY
specifi c primer identifi ed male specifi c 588, 333 and 200
bp fragments in males but only 200 bp fragment in females
Hemigrammus caudovittatus
David and Pandian (2006)
DMRT-1 specifi c primer identifi ed 237 and 300 bp fragments
in males but a 200 bp fragment in females
Takifugu rubripes
Cui et al. (2006)
Sex linked anonymous marker sequence suggested female
heterogamety
Clarias gariepinus
Kovacs et al. (2000)
Sex linked RAPD marker
CgaY1
(2.6 kb) present in males
and produced faint male specifi c band at low stringency in
Heterobranchus longifi lis. CgaY2
(458 bp) produced similar
hybridization pattern in both sexes of
C. gariepinus
,
C
.
macrocephalus
and
H. longifi lis
Oncorhynchus
spp
Du et al. (1993), Nakayama
et al. (1998), Devlin et al.
(2001)
Sex linked
GH
pseudogene identifi ed in
O. keta,
O. gorbuscha
and
O. kisutch
, but it is absent in
O. nerka, O. mykiss
and
O. clarki
O. masou
Zhang et al. (2001)
PCR based identifi cation of growth hormone pseudogene
GH
ψ in 93-98% of males and in a few females, whose
GH-
ψ
fragment was identical to that of males and shared 95%
homology with that in male
O. tshawytscha
O. tshawytscha
Nagler et al. (2004)
Real time quantitative PCR analysis detected the presence of
a single copy of growth hormone pseudogene
GH
p in males
but its absence in females
O. tschawytscha
Brunelli and Thorgaard
(2004)
AFLP analysis of purifi ed DNA of YY gynogenic ♂ and ♀
identifi ed OtY2 that was amplifi ed in ♂ but not in ♀. OtY2
androgenic male specifi c amplifi cation were present in
coho, chum and sockeye salmon but not in pink salmon and
rainbow trout
Seriola quinqueradiata
Fuji et al. (2010)
Using 71 microsatellite markers, selected from genetic
linkage map of female (ZW), detected the presence of sex
determining locus between
sequ21
and
sequ17
in LG12
Salvelinus namaycush
Stein et al. (2002)
Detection of sex linked microsatellite locus near the telomere
of Y chromosome
X. helleri
Kazianis et al.
(2004), Woolcock et al.
(2006)
Identifi cation of sex chromosomes by several molecular
markers and mapping of sex determining region by linkage
group 24
Poecilia reticulata
Kirpichnikov (1981), Khoo
et al. (1999a, b), Lindholm
et al. (2004), Nanda et
al. (1992), Tripathy et al.
(2009)
Traits like Black caudal peduncle (Bcp) and Red tail (
Rdt
)
markers linked to the dominant T chromosome. Traits like
Macropodus, Aramatus
and
Pauper
pigmentation linked to
YY chromosomes. Using (GATA)
4
and (GACA)
4
probes, sex
chromosomes were identifi ed. Genetic linkage map of the
genome using 790 single nucleotide polymorphism markers
Verasper variegattus
Ma et al. (2010)
V'eval F 533 and species specifi c V'eval 218 markers present
in females only
