Biology Reference
In-Depth Information
B
A
hybrid
C
Kaga
D
Transplanted
F
E
M
bp
1000
500
MFO1FSAO34MO1 MFO1FSAOO1A14
Fig. 34.
Recovery of fertility in sterile hybrid medaka by PGCs transplantation from fertile
medaka. A = medaka with arrow indicating egg being spawned, B = colonization of
'undifferentiated' sterile gonad by transplanted PGCs, C = Cross sections of ovaries from
a 4 week old Kaga strain , D = Non transplanted hybrid, E =Transplanted hybrid ovary
contains growing oocytes, as shown by arrows. Scale bar, 200 µm and F = Confi rmation of
germ-line transfer by detection of strain-specifi c genetic markers. Note that the patterns of
marker amplifi cation for the progeny are identical to those of the donor (from Shimada and
Takeda, 2008)
