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Fig. 10. Oryzias latipes : (A): PCR genotyping of males and females. Note the presence of
Dmrt1bY in normal males but its absence in aberrant females. (B): Hemizygosity of normal
males of the HB32C and HNI strains for caspase 6. Females and aberrant males (*) show only
the X chromosomal PCR product. (C): Southern blot analysis of DNA from female, normal male
and aberrant ( Dmrt1bY negative) males (*). FISH pattern of Y specifi c and sex chromosome
specifi c probes on (D) XY and (E) XX metaphase chromosomes. Note the presence of three
hybridization signals in XY males, as compared to two spots in XX males (from Nanda et al.,
2003, with permission by the Genetic Society of America).
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