Biology Reference
In-Depth Information
1
2
M C1 C2 1 2 3 4 5 6 7 M
M C1 C2 1 2 3 4 5 6 7 8 9 M
A
A
B
B
C
C
3
1
2
3
4
5
6
7
8
9
10
11
12
175 pb
125 pb
Recipients
Donors
Offspring
Mothers
Fig. 37.
(1): PCR analysis of sperm of 8-month old recipient
Odontesthes hatcheri.
(A) The donor
O. bonariensis
specifi c sequence, (B)
O. hatcheri
specifi c sequence, (C) β-actin control. Note the
donor-derived
O. bonariensis
spermatozoa in four recipients, as shown in lanes: 1, 2, 4 and 6.
(2): PCR analysis of progenies sired by allogenic
O. bonariensis/
surrogate crossed with normal
O. bonariensis
female (Note the donor-derived
O. bonariensis
spermatozoa in four recipients,
as shown in lanes: C
2
, 3, 7 and 8) (from Majhi et al., 2009). (3): Genomic DNA analysis of
Oreochromis niloticus
. Alleles from donors were detected in two progenies (rectangle), indicating
that these fi sh were derived from donor cells and were not related to the surrogate recipients
(from Lacerda et al. 2010)
these allogenic/xenogenic females suffered greater mortality (Table 24).
Incidentally, a reason for the wide differences between the allogenics and
xenogenics is that the cell suspension used for transplantation contained
56% gonial cells in the xenogenics, against 12% only in allogenics, and 2.
Both OSCs and SSCs have retained bisexual potency even after maturation
and milting/spawning by the respective donors but are allowed to express
their bisexual potency, so long as the recipients are alevins with sexually
undifferentiated gonad, i.e., gonadal differentiation leads to the reduction
of bisexual potency of germ cells supporting somatic cells into unisexual
