Biology Reference
In-Depth Information
OSCs: As indicated by Xu et al. (2005), the PGC-derived OSCs have
also retained sexual plasticity. Yoshizaki et al. (2010) intraperitoneally
transplanted 20-30 nl of ovarian cell suspension containing ≈ 15,000 cells with
12%
Gfp
-positive germ cells of the trout into 25 dph alevin. At 20 day post-
transplantation (dpt), 33 out of 55 recipients showed the presence of donor-
derived germ cells (Fig. 35A,A1), and the gonads of each of the
Gfp
-positive
recipients were colonized by 3.3 ± 2.6 germ cells. However, on the 150 dpt,
A1
A
A2
B
D
C
E
G
F
Fig. 35.
Colonization of recipient gonads by ovarian germ cells of
Oncorhynchus mykiss.
(A-A
1
)
perinuclear oocytes (PNO) and oocytes (arrow heads) showing
Gfp
—specifi c antibody. (A2)
Gfp
-labeled donor germ cells proliferated and differentiated into oocytes in recipient ovary. (B)
Gfp
-labeled donor-derived germ cells remaining in the post-ovulatory follicles of 3n recipient.
50% F
1
progenies were OSCs-derived orange colored females (C) and males (D). Fluorescent
(E) and bright-fi eld (F) views of colonization and proliferation of
Gfp
-labeled donor germ cells
in the testes of 5 month old recipients. (g) The percentage of progenies carrying the donor-
derived germ cells in their gonads (black bars) and those, who did not differ signifi cantly
between female recipients and male recipients (from Yoshizaki et al., 2010. Sexual plasticity of
ovarian germ cells in rainbow trout. Development, 137 : 1227-1230, doi : 10.1242/dev 044982,
reproduced with kind permission by The Company of Biologists Ltd)
Color image of this figure appears in the color plate section at the end of the topic.
