Biology Reference
In-Depth Information
B
A
hybrid
C
Kaga
D
Transplanted
F
E
M
bp
1000
500
MFO1FSAO34MO1 MFO1FSAOO1A14
Fig. 34.
Recovery of fertility in sterile hybrid medaka by PGCs transplantation from fertile
medaka. A = medaka with arrow indicating egg being spawned, B = colonization of
'undifferentiated' sterile gonad by transplanted PGCs, C = Cross sections of ovaries from a 4
week old Kaga strain, D = Non transplanted hybrid, E =Transplanted hybrid ovary contains
growing oocytes, as shown by arrows. Scale bar, 200 µm and F = Confi rmation of germ-line
transfer by detection of strain-specifi c genetic markers. Note that the patterns of marker
amplifi cation for the progeny are identical to those of the donor (from Shimada and Takeda,
2008)
Color image of this figure appears in the color plate section at the end of the topic.
males but continued to remain sterile. Notably, the transplantation of PGCs
into the recipients blastulae, that have 'undifferentiated' but sterile testes
and ovaries, let the PGCs to develop males and females at the ratio of
0.6 : 0.4. An ideal experiment, that is urgently required is the one, in which
the PGCs, SSCs or OSCs are transplanted into sterile ovaries of adult hybrid
recipient.
