Microvascular Permeability and Tumor Metastasis - Bioanalysis Advanced Materials Methods and Devices - page 56

Fig. 4.3 ( a ) 3D sketch of the junction-orifice-matrix entrance layer model for the interendothelial
cleft. 2B is the width of the cleft. Large junction breaks observed in Adamson et al. [ 1 ] are
2 d 2 B , while the small continuous slit in the junction strand is 2 b s .( b ) plane view of the model.
Junction strand with periodic openings lies parallel to the luminal front of the cleft. L 2 , depth of
pores in junction strand. L 1 and L 3 , depths between junction strand and luminal and abluminal
fronts of the cleft, respectively. 2 D , distance between adjacent large junction breaks. At the
entrance of the cleft on luminal side, cross-bridging structures are represented by a periodic square
array of cylindrical fibers. a, radius of these fibers,

, gap spacing between fibers, and L f , thickness
of entrance fiber layer. The fiber matrix (surface glycocalyx) carries negative charge due to its
molecular composition. The charge density of the surface glycocalyx is in the range of 20-30 mEq/
L for mesenteric and brain microvessels. Revised from Fu et al. [ 27 - 29 ], Li and Fu [ 46 ], and Yuan
et al. [ 88 ]

D