Inertial Migration of Cancer Cells in a Microfluidic Device - Bioanalysis Advanced Materials Methods and Devices - page 26

When d 0.5. Therefore, half of the cancer cells have a migration

length L m greater than that of the rigid spheres. By substituting d 0 ¼

¼ d 0 , CDF ¼

15 m m and

s ¼ m into ( 2.5 ), it can be shown that for 18% of the cancer cells, the

migration length is more than double that of the rigid spheres.

4.3 m

2.7 Conclusion

In this chapter we introduce our recent study of inertial migration of cancer cells in
a microchannel [ 22 ]. The basic mechanism of inertial migration, fabrication of a
PDMS microchannel, and the cancer cell preparation are all described. The inertial
migration of both rigid spheres and cancer cells was convincingly observed in the
microchannel. The migration of cancer cells in a dilute suspension was not as strong
as that of rigid spheres, and the channel length required for cancer cell migration
was roughly double that for the rigid spheres. The low efficiency of cell migration
was attributable mainly to the size variation of the cancer cells. These results have
important implications for the design of a microfluidic device for separating
targeted cells from other cells.

Acknowledgments This study was supported by Grants-in-Aid for Scientific Research (S) from
the Japan Society for the Promotion of Science. We also acknowledge support from the 2007
Global COE Program “Global Nano-Biomedical Engineering Education and Research
Network Centre.”

References

1. Bhagat AAS, Kuntaegowdanahalli SS, Papautsky I (2009) Inertial microfluidics for continuous
particle filtration and extraction. Microfluid Nanofluid 7:217-226
2. Bhagat AAS, Bow H, Hou HW et al (2010) Microfluidics for cell separation. Med Biol Eng
Comput 48:999-1014
3. Budd GT, Cristofanilli M, Ellis MJ et al (2006) Circulating tumor cells versus imaging-
predicting overall survival in metastatic breast cancer. Clin Cancer Res 12:6403-6409
4. Carlo DD, Edd JF, Irimia D et al (2008) Equilibrium separation and filtration of particles using
differential inertial focusing. Anal Chem 80:2204-2211
5. Carlo DD (2009) Inertial microfluidics. Lab Chip 9:3038-3046
6. Cristofanilli M, Budd GT, Ellis MJ et al (2004) Circulating tumor cells, disease progression,
and survival in metastatic breast cancer. N Engl J Med 351:781-791
7. Cristofanilli M, Hayes DF, Budd GT et al (2005) Circulating tumor cells: a novel prognostic
factor for newly diagnosed metastatic breast cancer. J Clin Oncol 23:1420-1430
8. Fujiwara H, Ishikawa T, Lima R et al (2009) Red blood cell motions in high-hematocrit blood
flowing through a stenosed microchannel. J Biomech 42:838-843
9. Hur SC, Tse HTK, Carlo DD (2010) Sheathless inertial cell ordering for extreme throughput
flow cytometry. Lab Chip 10:274-280