Method Development, Validation, and Sample Analysis for Regulated Quantitative Bioanalysis Using LC-MS/MS - LC-MS in Drug Bioanalysis - page 45

Fig. 5 Armodafinil computational results from ACD LC Simulator using Pentanol as mobile B
( top panel : 0.4 % Pentanol 2 × 50 mm AGP; middle panel , 0.2 % Pentanol 2 × 50 mm AGP; bottom
panel : 0.4 % Pentanol 2 × 100 mm AGP)

software, it cannot be relied upon as a sole MD strategy. The software only works
with the imported chromatograms and mobile phase provided by the user. As a
result, due to these limitations it can only serve as a method development aid. Sound
scientific judgment and laboratory experiment is still required to select the appropri-
ate starting condition. Using this approach, we successfully developed four chiral
assays such as eszopiclone, armodafinil, ramelteon, and dexlansoprazole [ 11, 12 ] .

4 Considerations While Developing LC-MS/MS Methods

4.1 Phospholipids Related Matrix Effect

The focus in early quantitative LC-MS/MS analysis was on recovery, simplicity of
extraction, and fastness. Researchers gradually discovered and began to report the