Biomedical Engineering Reference
In-Depth Information
Sample Name: "1 015 TSLS10018 std 1 1 1" Sample ID: "15" File: "S1001801.wiff"
Peak Name: "Raltegravir" Mass(es): "443.2/316.1 amu"
Comment: "none" Annotation: ""
Sample Index: 28
Sample Type: Standard
Concentration: 1.0000 ng/mL
Calculated Conc: 0.97296 ng/mL
Acq. Date: 1/25/2010
Acq. Time: 1:19:34 PM
Modified: No
Proc. Algorithm: IntelliQuan - MQII
Noise Percentage: 40
Base. Sub. Window: 1.00 min
Peak-Split. Factor: 3
Report Largest Peak: No
Min. Peak Height: 150.00 cps
Min. Peak Width: 0.00 sec
Smoothing Width: 0 points
RT Window: 30.0 sec
Expected RT: 0.767 min
Use Relative RT: No
Int. Type: Base To Base
Retention Time: 0.742 min
Area: 2773.35987 counts
Height: 740.000 cps
Start Time: 0.662 min
End Time: 0.847 min
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0
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0.2
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1.0
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1.6
Time, min
Sample Name: "1 015 TSLS10018 std 1 1 1" Sample ID: "15" File: "S1001801.wiff"
Peak Name: "25_Ralt-13C6(IS)" Mass(es): "451.2/324.1 amu"
Comment: "none" Annotation: ""
Sample Index: 28
Sample Type: Standard
Concentration: 1.00 ng/mL
Calculated Conc: N/A
Acq. Date: 1/25/2010
Acq. Time: 1:19:34 PM
Modified: No
Proc. Algorithm: IntelliQuan - IQA
Min. Peak Height: 150.00 cps
Min. Peak Width: 0.00 sec
Smoothing Width: 0 points
RT Window: 30.0 sec
Expected RT: 0.872 min
Use Relative RT: No
Int. Type: Valley
Retention Time: 0.756 min
Area: 1013219.62373 counts
Height: 198000. cps
Start Time: 0.511 min
End Time: 0.991 min
0.76
1.9e5
1.8e5
1.7e5
1.6e5
1.5e5
1.4e5
1.3e5
1.2e5
1.1e5
1.0e5
9.0e4
8.0e4
7.0e4
6.0e4
5.0e4
4.0e4
3.0e4
2.0e4
1.0e4
0.0
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1.0
1.1
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1.6
Time, min
F i g . 2
Representative LLOQ of raltegravir (1 ng/mL) using online SPE-API 4000. HySphere™ C18HD 7 mm cartridge was utilized and there was no analyti-
cal column
