Biomedical Engineering Reference
In-Depth Information
Sample Name: "1 011 S09081 std 2 1 1" Sample ID: "11" File: "S0908101.wiff"
Peak Name: "Raltegravir" Mass(es): "443.4/316.1 amu"
Comment: "none" Annotation: ""
Sample Index: 27
Sample Type: Standard
Concentration: 2.00 ng/mL
Calculated Conc: 16.1 ng/mL
Acq. Date: 11/19/2009
Acq. Time: 6:35:06 PM
Modified: No
Proc. Algorithm: IntelliQuan - IQA
Min. Peak Height: 400.00 cps
Min. Peak Width: 2.00 sec
Smoothing Width: 0 points
RT Window: 30.0 sec
Expected RT: 0.960 min
Use Relative RT: No
Int. Type: Base To Base
Retention Time: 0.960 min
Area: 2177 counts
Height: 880.000 cps
Start Time: 0.900 min
End Time: 1.047 min
0.96
850
800
750
700
650
600
550
500
450
1.00
400
350
300
250
0.92
200
0.29
150
1.08
1.72
2.27
0.89
1.62
0.25
1.20
2.54
2.72
2.78
100
1.10
1.98
0.49
2.15
2.86
0.21
1.27
1.48
2.89
50
0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
Time, min
Sample Name: "1 011 S09081 std 2 1 1" Sample ID: "11" File: "S0908101.wiff"
Peak Name: "Raltegravir-13C6(IS)" Mass(es): "451.0/324.0 amu"
Comment: "none" Annotation: ""
Sample Index: 27
Sample Type: Standard
Concentration: 1.00 ng/mL
Calculated Conc: N/A
Acq. Date: 11/19/2009
Acq. Time: 6:35:06 PM
Modified: No
Proc. Algorithm: IntelliQuan - IQA
Min. Peak Height: 500.00 cps
Min. Peak Width: 5.00 sec
Smoothing Width: 0 points
RT Window: 30.0 sec
Expected RT: 0.956 min
Use Relative RT: No
Int. Type: Valley
Retention Time: 0.956 min
Area: 1340694 counts
Height: 358000. cps
Start Time: 0.806 min
End Time: 1.26 min
0.96
3.4e5
3.2e5
3.0e5
2.8e5
2.6e5
2.4e5
2.2e5
2.0e5
1.8e5
1.6e5
1.4e5
1.2e5
1.0e5
8.0e4
6.0e4
4.0e4
2.0e4
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
Time, min
F i g . 1
Representative LLOQ of raltegravir (1 ng/mL) using offline SPE-API 5000. Strata-X 96-well plate and a Waters Xbridge™ phenyl 2 × 50 mm column
was utilized
