Biomedical Engineering Reference
In-Depth Information
Table 5
(conti
nued)
Ionization/mass
analyser/scan mode Reference
Tio SB-C18 Zorbax (250 × 4.6)/0.6/22 40 mM NH
4
OAc/MeOH 56:44 (iso) 0.0015-0.03 +ESI/SQ/SIM [
81
]
Trop Diamonsil C
18
(150 × 4.6)/0.5/25 MeOH/H
2
O 80:20, 0.2 % FA (iso) 0.1-100 +APCI/QqQ/MRM [
83
]
Tros HyPurity C
18
(50 × 4.6)/0.5/24 ACN/2 mM NH
4
OAc 80:20 (iso) 0.05-10 +ESI/QqQ/MRM [
84
]
For abbreviation of analyte names see Sect. “Abbreviations”.
ACN
acetonitrile,
APCI
atmospheric pressure chemical ionization,
dial.
microdialysis samples,
ESI
electrospray ionization,
FA
formic acid,
iso
isocratic,
IT
ion trap,
lin range
linear range,
MeOH
methanol,
MRM
multiple reaction monitoring,
MS
full scan
mass spectrometry,
n.s.
not speci fi ed,
OAc
acetate,
QqQ
triple quadrupole mass spectrometer,
SIM
selected ion monitoring,
Solv
HPLC solvent,
SQ
single
quadrupole mass spectrometer,
T
temperature
a
Ratios given as v/v
b
Lower limit of linear range corresponds to lower limit of quantification
c
Analysis of equine urine
d
Analysis of human serum and microdialysate
Analyte
Column (mm × mm)/ fl ow (ml/min)/
T
(°C) Mobile phase
a
(run mode)
Lin range (ng/ml)
b
