Biomedical Engineering Reference
In-Depth Information
Table 3
Preparation of mammalian samples by liquid-liquid extraction (LLE) prior to LC-MS analysis
Sample:solvent
volume ratio
b
Organic solvent
a
Sample matrix
Analyte
Steps prior to LLE
Recovery (%)
Reference
C
5
H
12
/H
2
CCl
2
/
iso
prop
46:49:5
Rat urine, bile
Benz
n.s. (3×)
Lyophilization, +MeOH +PB, pH 7.2
c
n.s.
[
59
]
HCCl
3
Liver microsomes
d
Cim
1:1.4 (2×)
+IS +NaCl +hepSA, pH 3.7
75
[
23
]
H
2
CCl
2
Hum plasma
S
-Hyo
1:4.3
+IS +1 M NH
4
OH
110
[
90
]
H
2
CCl
2
Hum plasma
Cim
1:11.8
+IS
64
[
61
]
H
2
CCl
2
Hum plasma
BuS
1:8.6
+IS
69
[
79
]
H
2
CCl
2
Rat, mouse, hamster,
guinea pig liver
microsomes
Cim, metab.
1:1.4 (2×)
+IS +NaCl +hepSA, pH 3.7
62
[
23
]
H
2
CCl
2
Hum plasma
Tio
1:2
+IS +ACN (precipitation)
e
76-81
[
80
]
H
2
CCl
2
/
iso
prop 4:1
Equine urine
Atr, Scp
1:0.73
+1 M NaOAc pH 5.0 +b-glucu +25 %
NH
4
OH
f
n.s.
[
14
]
EE
Liver microsomes
d
Cim
1:1.4 (2×)
+IS +NaCl +hepSA, pH 3.7
32
[
23
]
EE
Rat faeces
Ada, metab.
1:2
+0.001 % Na
2
CO
3
n.s.
[
6
]
EE
Rat liver homogenate
Ada, metab.
1:2
n.s.
[
6
]
EE
Rat faeces
Adi, metab.
1:2
+0.001 % Na
2
CO
3
n.s.
[
5
]
EE
Rat liver homogenate
Adi, metab.
1:2
n.s.
[
5
]
EE
Rat faeces
Atr, metab.
1:2
+0.001 % Na
2
CO
3
n.s.
[
52
]
EE
Rat faeces
Scp
1:2
+0.001 % Na
2
CO
3
n.s.
[
87
]
EE
Equine urine
Ipra
1:1
+IS +borax buffer +5 M NaOH, pH 10.0
g
82
[
24
]
EE
Hum plasma
Gran
1:6.3
+IS +0.1 M NaOH
98
[
71
]
EE/C
6
H
14
75:25
Dog, monkey urine
Beme (MDL
72,222), metab.
n.s. (2×)
Lyophilization, +acid, pH 2.0 or +base,
pH 10.0
n.s.
[
37
]
Et
2
O
Liver microsomes
d
Cim
1:1.4 (2×)
+IS +NaCl +hepSA, pH 3.7
54
[
23
]
