Biomedical Engineering Reference
In-Depth Information
Table 2
Preparation of mammalian samples by precipitation prior to LC-MS analysis
Sample:solvent
volume ratio
Precipitation solvent
a
Sample matrix
Analyte
Comment
Recovery (%)
Reference
ACN
Hum plasma
Atr
1:1.5
Solvent containing IS
38-41
[
44
]
ACN
Hum plasma
Atr, Coc, Hatr, Ipra, Lit,
BuS, Scp
1:2
+IS a.p.
93, 94, 86, 90, 93,
92, 88
[
50
]
ACN Hum plasma
R
/
S
-Hyo 1:2 +AtrE p.p. +IS a.p.
b
93 [
49
]
ACN Porcine plasma
R
/
S
-Hyo 1:2 +AtrE p.p. +IS a.p.
b
93 [
47
]
ACN Rabbit plasma
R
/
S
-Hyo 1:2 93 [
49
]
ACN Rat plasma Gran 1:1.1 +IS p.p. > 100 [
72
]
ACN/MeOH 5:1 Hum serum Atr 1:7.5 Solvent containing IS n.s. [
53
]
Chlorobutane Rabbit plasma Scp 1:2 n.s. [
89
]
MeOH Rat plasma Adi, metab. 1:3 n.s. [
5
]
MeOH Rat plasma Ada, metab. 1:3 n.s. [
6
]
MeOH Rat plasma Atr, metab. 1:3 n.s. [
52
]
MeOH Rat plasma Scp, metab. 1:3 n.s. [
87
]
MeOH Hum liver microsomes Trop, metab. 1:1 n.s. [
82
]
MeOH Rat plasma Sat 1:2.7 +IS p.p. 76-85 [
85
]
ZnSO
4
aq 1 % w/v Liver microsomes
c
Cim, metab. 1:0.3 +IS p.p., SPE a.p. 100 [
23,
62
]
For abbreviation of analyte names see Sect. “Abbreviations”.
ACN
acetonitrile,
a.p.
after precipitation,
AtrE
atropinesterase from rabbit serum,
hum
human,
IS
internal standard,
metab.
metabolites (biotransformation products produced in vivo),
MeOH
methanol,
n.s.
not speci fi ed,
p.p.
prior to precipitation
a
Numbers give volume ratio v/v
b
AtrE was added to hydrolyze
S
-hyoscyamine selectively for enantioselective analysis
c
Microsomes from rat, mouse, hamster, guinea pig
