Biomedical Engineering Reference
In-Depth Information
Table 4
(continued)
Method sensitivity, LOQ (S/N ³ 5)
GC/NCI/
MS/MS
LC/APCI
+
/
MS/MS
LC/APCI
−
/
MS/MS
LC/ESI
−
/
MS/MS
LC/ESI
+
/
MS/MS
LC/ESI
+
/
MS/MS
LC/APPI
+
/
MS/MS
LC/APPI
−
/
MS/MS
GC/MS
Serum,
1.0 mL
Prostatic
tissue
Serum,
0.5 mL
Milk,
10 mL
Urine,
0.2 mL
Serum,
0.2 mL
Serum,
0.2 mL
Urine, 2 mL
Serum
carbonyl
derivative
(ng/g)
(LOD)
Underiva-
tized
(ng/L)
(LOD)
MSTFA
derivatives
(ng/mL)
PFB,
MSTFA
(pg/mL)
Underiva-
tized
(pg/L)
Underiva-
tized
(ng/mL)
NH
2
OH
derivative
(pg/mL)
Underiva-
tized
(pg/mL)
Underiva-
tized
(pg/mL)
Compound
Abbreviation FW
Tetrahydrocor ticosterone
THB
350
Tetrahydrodeoxycortisol
THS
350
5,000
Cortisone E
360
5,000
Cortisol F
362
0.3
20 a -Dihydrocortisone
20 a -DHE
362
20 a -Dihydrocortisol
20 a -DHF
364
Allodihydrocortisol
Allo-DHF
364
Tetrahydrocortisone
THE
364
Tetra-11-dehydrocor-
ticosterone
THA
364
a -Cortolone
366
b -Cortolone
366
Tetrahydrocortisol
THF
366
1
Allotetrahydrocortisol
Allo-THF
366
3
a -Cortol
368
3
b -Cortol
368
Sterols
Cholestenone
384
Desmolesterol
384
