Biomedical Engineering Reference
In-Depth Information
Table 4
(continued)
Method sensitivity, LOQ (S/N ³ 5)
GC/NCI/
MS/MS
LC/APCI
+
/
MS/MS
LC/APCI
−
/
MS/MS
LC/ESI
−
/
MS/MS
LC/ESI
+
/
MS/MS
LC/ESI
+
/
MS/MS
LC/APPI
+
/
MS/MS
LC/APPI
−
/
MS/MS
GC/MS
Serum,
1.0 mL
Prostatic
tissue
Serum,
0.5 mL
Milk,
10 mL
Urine,
0.2 mL
Serum,
0.2 mL
Serum,
0.2 mL
Urine, 2 mL
Serum
carbonyl
derivative
(ng/g)
(LOD)
Underiva-
tized
(ng/L)
(LOD)
MSTFA
derivatives
(ng/mL)
PFB,
MSTFA
(pg/mL)
Underiva-
tized
(pg/L)
Underiva-
tized
(ng/mL)
NH
2
OH
derivative
(pg/mL)
Underiva-
tized
(pg/mL)
Underiva-
tized
(pg/mL)
Compound
Abbreviation
FW
2-Hydroxyestrone
2-OH-E1
286
0.2
14
<8>
4-Hydroxyestrone
4-OH-E1
286
0.2
50
<8>
16-Keto-17 b -estradiol
16-keto-E2
286
0.2
4,200
<8>
16 a -Hydroxyestrone
16 a -OH-E1
286
0.2
4,800
<8>
2-Hydroxy-17 b -estradiol
2-OH-E2
288
0.2
14
<8>
4-Hydroxy-17 b -estradiol
4-OH-E2
288
0.2
30
17-Epiestriol
17-epi-E3
288
1.0
<8>
Estriol
E3
288
0.5
15,700
50
3
1 (8)
16-Epiestriol
16-epi-E3
288
1.0
6,600
<8>
4-Methoxyestrone
4-MeO-E1
300
8,700
<8>
4-Methoxy-17 b -estradiol
4-MeO-E2
302
0.5
19,200
<8>
2-Methoxyestrone
2-MeO-E1
300
0.1
32,400
<8>
3-Methoxyestrone
3-MeO-E1
300
<8>
2-Methoxy-17 b -estradiol
2-MeO-E2
302
0.1
61,500
<8>
2-Hydroxyestriol
2-OH-E3
304
0.5
Progestagens
Progesterone
Prog
314
1.0
0.3
50
5 a -Dihydroprogesterone
5 a -DHP
316
0.5
5 b -Dihydroprogesterone
5 b -DHP
316
0.2
