Biomedical Engineering Reference
In-Depth Information
Table 4
Comparison of GC-MS, GC-MS/MS, and LC-MS-MS in separation and sensitivity
Method sensitivity, LOQ (S/N ³ 5)
GC/NCI/
MS/MS
LC/APCI
+
/
MS/MS
LC/APCI
−
/
MS/MS
LC/ESI
−
/
MS/MS
LC/ESI
+
/
MS/MS
LC/ESI
+
/
MS/MS
LC/APPI
+
/
MS/MS
LC/APPI
−
/
MS/MS
GC/MS
Serum,
1.0 mL
Prostatic
tissue
Serum,
0.5 mL
Milk,
10 mL
Urine,
0.2 mL
Serum,
0.2 mL
Serum,
0.2 mL
Urine, 2 mL
Serum
carbonyl
derivative
(ng/g)
(LOD)
Underiva-
tized
(ng/L)
(LOD)
MSTFA
derivatives
(ng/mL)
PFB,
MSTFA
(pg/mL)
Underiva-
tized
(pg/L)
Underiva-
tized
(ng/mL)
NH
2
OH
derivative
(pg/mL)
Underiva-
tized
(pg/mL)
Underiva-
tized
(pg/mL)
Compound
Abbreviation FW
Androgens
Androstenedione
A-dione
286
2.0
Dehydroepiandrosterone
DHEA
288
50.0
1
Androstanedione
5 a -dione
288
5.0
Epitestosterone
Epi-T
288
0.1
0.3
10
Testosterone
T
288
0.1
0.3
10
5 b -Dihydrotestosterone
5 b -DHT
290
0.2
50*
Androsterone
An
290
0.5
1
Etiocholanolone
Etio
290
0.5
1
Epietiocholanolone
Epi-Etio
290
1
Epiandrosterone
Epi-An
290
5.0
1
Androstenediol
A-diol
290
2.0
<0.35
5 a -Dihydrotestosterone
5 a DHT
290
0.2
0.5
50*
5 b -Androstan-3 a ,
17a -diol
b a a -diol
292
1.0
200
5 b -Androstan-3 b ,
17a -diol
b b a -diol
292
0.5
200
5 a -Androstan-3 a ,
17a -diol
a a a -diol
292
