Biomedical Engineering Reference
In-Depth Information
Table 2
Typical steroid hormone sample preparation techniques
Hydrolyzing
conjugates
Steroids in matrix
Extraction
Derivatization
Internal standard
MS mode
Reference
Estrogens in serum
Steroids in testicular fluid
NA
Solvent precipitation
vs. LE
LLE
NA
Deuterated isotopes
LC-APPI
−/+
/MS/MS
LC-ESI
+
/MS/MS
[
22
]
[
24
]
Estrogens in breast tissue,
plasma, brain
NA
Solvent precipitation/LLE
LLE
Hydroxylamine
Dansyl chloride
Deuterated isotopes
LC-TIS
+
/MS/MS
LC-ESI
+
/MS/MS
LC-ESI
+
/MS/MS
[
25
]
[
26
]
[
27
]
Steroids in urine
Estrogens in urine, serum
b -Glucuronidase/
H
2
SO
4
b -Glucuronidase/
sulfatase
LLE
LLE
NA
Dansyl chloride
Deuterated isotopes
LC-ESI
+
/MS/MS
[
20
]
[
8,
28
]
Estrones in cell culture
medium
Steroids in water
NA
SPE (96-well C
18
)
NA
Deuterated isotopes
NA
LC-APCI
+/−
/MS/MS vs.
LC-ESI
+/−
/MS/MS
LC-ESI
−
/MS/MS
[
29
]
[
30
]
Magnetic-mediated SPE
Androgens in prostatic tissue
Androgens in plasma
Estrogens in sediment and
sewage sludge
Estrogens in water
NA
SPE (96-well Oasis HLB)
Sep-Pak C
18
Sep-Pak C
18
2-Fluoro-1-
methypyridinium
p
-toluenesulfonate
(FPMTS)
Penta fl uoropropionic
anhydride
BSTFA
MSTFA
Deuterated isotopes
LC-ESI
+
/MS/MS
GC-EI
+
/MS
LC-ESI
+
/MS and GC-EI
+
/
MS
GC-EI
+
/MS
[
31
]
[
32
]
[
33
]
[
34
]
Supelco Discovery C
18
Estrones in urine
b -Glucuronidase/
sulfatase
SPE (Bobd Elut LRC C
18,
Extrlut QE)
p
-Toluenesulfonhydrazide
Deuterated isotopes
LC-ESI
+
/MS and
LC-APCI
+
/MS,
LC-EI
+
/MS
n
[
35,
36
]
Estrogens in urine
b -Glucuronidase
SPE (Sep-pak C18,
Sephadex A25,
Oasis HLB)
MSTFA
Deuterated isotopes
GC-EI
+
/MS
[
37,
38
]
Steroids in urine
b -Glucuronidase/
arylsulfatase
bCD (Oasis HLB)
GC-EI
+
/MS
[
6,
39
]
NA
, not applicable
