Biomedical Engineering Reference
In-Depth Information
Plasma
b
(200 m l)
Tam
N -D-Tam
4-OH-Tam
Endoxifen
Tam-d5
N -D-Tam-d5
4-OH-Tam-d5
Endoxifen-d5
d
1.86
1.78
0.194
0.187
PP (ACN/acetone
(1:1))
LLE ( n -hexane/
isopropanol
(95:5))
Evaporation
Reconstitution (ACN/
H
2
O/formic acid
(40:60:0.1 v/v/v))
Centrifugation
Acquity UPLC BEH
C18 (1.7 m m,
100 × 2.1 mm)
ESI-TSQ
[
247
]
Plasma
(100 m l)
Tam
N -D-Tam
4-OH-Tam
Endoxifen
Imipramine
20
40
1
4
PP (ACN)
Acquity UPLC BEH
C18 (1.7 m m,
100 × 2.1 mm)
ESI-TSQ
[
227
]
a
Non-clinical samples
b
Fully validated method
c
Method has not been validated for the quantification of the analyte
d
Reported E/Z isomers chromatographic resolution
e
Abbreviations (see Fig.
2
and Table
4
)
