Biomedical Engineering Reference
In-Depth Information
Table 3
Overview of LC-MS and LC-MS/MS methods for the quantification of tamoxifen and its metabolites in human blood samples
LOQ
(ng/ml)
Column (particle
size, dimensions)
Ionization and
detection mode
Matrix (volume)
Analytes
e
Internal standard
Sample preparation
Reference
Plasma
a
(100 m l)
Tam
4-OH-Tam
Idoxifene-d5
5
-
LLE (hexane/
isoamyl-alcohol
(96:4 v/v))
Dilution (DMSO)
Evaporation (hexane
layer)
Dilution (H20)
Luna C18 (3 m m,
30 × 1 mm)
ESI-TSQ
[
235
]
Others SERMS
(raloxifene,
nafoxidine,
idoxifene)
Plasma
(250 m l)
Tam
N-D-Tam
4-OH-Tam
Toremifene
-
PP (ACN)
Dilution (0.5 M
ammonium
acetate)
Hypersil BDS C18
(3 m m,
150 × 2.1 mm)
ESI-Q-TOF (MS
mode)
[
236,
237
]
Serum
b
(75 m l)
Tam
4-OH-Tam
N -D-Tam
N -D-D-Tam
Tam-NO
Endoxifen
c
Tam-d5
0.25
0.25
0.25
1.0
1.0
-
PP (ACN)
On-line SPE (Oasis
HLB, 50 × 1 mm)
Chromolith
Performance,
RP-18e
(100 × 4.6 mm)
ESI-LTQ
[
193,
238
]
Serum
b
(100 m l)
Tam
N -D-Tam
4-OH-Tam
Endoxifen
d
Soy iso fl avone
(genistein,
daidzein,
equol)
Tam-
13
C
2
,
15
N
N -D-Tam-d5
4-OH-Tam-d5
Endoxifen-d5
d
0.4
0.4
0.2
1.1
PP (ACN)
Hydrolysis
(glucuronidase/
sulfatase)
SPE (SPEC
96-WELL
PLATE C18)
Evaporation
Reconstitution
(MeOH/H2O
(1:1 v/v))
Luna C18 (3 m m,
150 × 2 mm)
ESI-TSQ
[
239,
240
]
(genistein-d4,
daidzein-d3,
equol-d4)
(continued)
