Biomedical Engineering Reference
In-Depth Information
Table 1
Selected LC-MS(MS) methods for antidepressants analysis in serum, plasma, and whole blood
IM/analyzer
(detection
mode)
Author, year [Ref]
Compounds
IS
Sample
Extraction procedure
Stationary phase
Mobile phase
Method validation
Zhang et al.
2000 [
58
]
Doxepine, imipramine,
desipramine, amitriptyline,
trimipramine
Imipramine-d3
Plasma (1 mL)
LLE: hexane
SB-C18 Mac Mod
rapid resolution
(15 × 2.1 mm, 3 m m)
3 mM
ammonium
acetate
pH 3.3/ACN
ESI+/TOF
(full scan)
-
Selectivity
-
LLOQ = 1-2 ng/mL
-
Linearity: 1/2 to
50/100 ng/mL;
r
2
> 0.99
Intra-/inter-day
imprecision: %CV < 18%
-
Intra-/inter-day
inaccuracy: MRE ± 15%
-
Relative recovery >69%
Kollroser et al.,
2002 [
74
]
Amitriptyline, melitracem,
dibenzepin, doxepin,
dusolepin, opipramol,
nortriptyline
lofepramine
Plasma (1 mL)
Online preparative
column: Oasis
HLB (50 × 1 mm,
30 m m)
Symmetry C18
(150 × 3 mm, 5 m m)
Symmetry C18 guard
column (20 × 3.9 mm,
5 m m)
Formic acid
0.1%/ACN
APCI+/IT
(daughter
full scan,
m
/
z
100-450)
-
LOD = 5 ng/mL
-
LLOQ = 10-50 ng/mL
-
Linearity: 10/50 to
800/1500 ng/mL;
r
2
> 0.99
-
Intra-/inter-day
imprecision: CV <15%
-
Intra-/inter-day
inaccuracy: MRE ± 15%
-
Absolute recovery >90%
-
Application to real
specimens
Kollroser et al.,
2003 [
75
]
Citalopram, fl uvoxamine,
paroxetine
Plasma (1 mL)
Online preparative
column: Oasis
HLB (50 × 1 mm,
30 m m)
Symmetry C18
(150 × 3 mm, 5 m m)
Symmetry C18 guard
column (20 × 3.9 mm,
5 m m)
Formic acid
0.1%/ACN
APCI+/IT
(daughter
full scan,
m
/
z
100-400)
-
Selectivity
-
LOD = 5 ng/mL
-
LLOQ = 10-20 ng/mL
-
Linearity: 10/20 to
600/800 ng/mL;
r
2
> 0.99
-
Intra-/inter-day
imprecision: CV <15%
-
Intra-/inter-day
inaccuracy: MRE ± 15%
-
Absolute recovery >86%
-
Matrix effect: %CV <10%
(
n
= 6)
(continued)
