Biomedical Engineering Reference
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Fig. 11 Typical chromatograms showing injection volume (I.V.) optimization for pooled serum
spiked with 100 pg/mL of 1,25(OH)2VitD3 and extracted by selective SPE. The I.V. was
( a ) 0.2 m L; ( b ) 0.6 m L; ( c ) 3 mL; and ( d ) 9.5 mL. A 15 cm × 0.5 mm I.D. capillary column (manu-
facturer suggestion I.V. was 0.2-0.4 mL) was used for separation. Using the selective SPE and a
sample focusing approach before the separation, a large I.V. was enabled without compromising
chromatographic performance and with substantially increased sensitivity (Reproduced with
permission from American Chemical Society)
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