Biomedical Engineering Reference
In-Depth Information
Fig. 9
Optimization of sample injection volume. Various injection volumes (
V
inj
) of spiked cell
lysate samples were investigated to optimize
V
inj
. Lysates were spiked with 250 pg/mL of paclitaxel
and extracted by selective SPE. The
V
inj
was (
a
) 0.1 m L; (
b
) 0.5 m L; (
c
) 2.0 m L; (
d
) 8.0 m L. A
15 cm × 0.5 mm I.D. capillary column was used for separation; the manufacturer-recommended
V
inj
for the column was 0.1-0.2 m L (Reproduced with permission from Elsevier)