Biomedical Engineering Reference
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Fig. 9 Optimization of sample injection volume. Various injection volumes ( V inj ) of spiked cell
lysate samples were investigated to optimize V inj . Lysates were spiked with 250 pg/mL of paclitaxel
and extracted by selective SPE. The V inj was ( a ) 0.1 m L; ( b ) 0.5 m L; ( c ) 2.0 m L; ( d ) 8.0 m L. A
15 cm × 0.5 mm I.D. capillary column was used for separation; the manufacturer-recommended V inj
for the column was 0.1-0.2 m L (Reproduced with permission from Elsevier)
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