Biomedical Engineering Reference
In-Depth Information
Fig. 5
Typical chromatograms showing injection volume (
V
i
nj
) optimization for plasma samples
spiked with 50 pg/mL of TACA and DEX-AC and extracted by selective SPE. The
V
inj
was (
a
) 0.1 m L;
(
b
) 0.5 m L; (
c
) 2 m L; (
d
) 5 mL. A 15 cm by 300 mm I.D. capillary column was used for separation; the
manufacturer-recommended
V
inj
was 0.1-0.2 mL. Selective SPE and two-segment gradient separation
enabled a large
V
inj
on a 300 mm I.D. capillary column without deterioration of chromatographic
performance and with greatly increased sensitivity (Reproduced with permission from American
Chemical Society)
